Inhibition of NHE protects reoxygenated cardiomyocytes independently of anoxic Ca overload and acidosis
نویسندگان
چکیده
Schäfer, C., Y. V. Ladilov, M. Schäfer, and H. M. Piper. Inhibition of NHE protects reoxygenated cardiomyocytes independently of anoxic Ca overload and acidosis. Am J Physiol Heart Circ Physiol 279: H2143–H2150, 2000.—We investigated the question of whether inhibition of the Na/H exchanger (NHE) during ischemia is protective due to reduction of cytosolic Ca accumulation or enhanced acidosis in cardiomyocytes. Additionally, the role of the Na-HCO3 2
منابع مشابه
Inhibition of NHE protects reoxygenated cardiomyocytes independently of anoxic Ca(2+) overload and acidosis.
We investigated the question of whether inhibition of the Na(+)/H(+) exchanger (NHE) during ischemia is protective due to reduction of cytosolic Ca(2+) accumulation or enhanced acidosis in cardiomyocytes. Additionally, the role of the Na(+)-HCO(3)(-) symporter (NBS) was investigated. Adult rat cardiomyocytes were exposed to simulated ischemia and reoxygenation. Cytosolic pH [2', 7'-bis(2-carbox...
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Isolated cardiomyocytes from adult rats were incubated in anoxic bicarbonate-buffered media at extracellular pH (pH(o)) 6.4 until a cytosolic Ca(2+) overload and intracellular pH (pH(i)) of 6.4 were reached. On reoxygenation, the pH of the medium was changed to 7.4 to activate the Na(+)/H(+)exchanger (NHE) and the Na(+)-HCO(-)(3) symporter (NBS). The reoxygenation was performed in the absence o...
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Schäfer, C., Y. V. Ladilov, B. Siegmund, and H. M. Piper. Importance of bicarbonate transport for protection of cardiomyocytes against reoxygenation injury. Am J Physiol Heart Circ Physiol 278: H1457–H1463, 2000.—Isolated cardiomyocytes from adult rats were incubated in anoxic bicarbonate-buffered media at extracellular pH (pHo) 6.4 until a cytosolic Ca21 overload and intracellular pH (pHi) of ...
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